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1.
Sci Rep ; 14(1): 7497, 2024 Mar 29.
Artigo em Inglês | MEDLINE | ID: mdl-38553522

RESUMO

Reducing recoil as well as reducing muzzle hazards are important issues in artillery design. This paper presents a barrel gas reflection method for the artillery aiming for efficient recoil reduction while reducing muzzle hazards. The launching process is modeled by coupling the interior ballistic equations and the flow equations of the barrel gas reflection device. The fourth-order Runge-Kutta method was used to solve the model, and the LHS method as well as the Kriging model was used to establish a mapping relationship between the parameters and the effect. To validate the proposed model, shooting experiments are carried out on a 30 mm caliber artillery. The maximum error between the experiment and simulation results was 5.32%. The experiment has demonstrated that the barrel gas reflection method increases the recoil reduction efficiency of artillery by 44.54% and reduces the muzzle hazard by 52.18%. Finally, the barrel gas reflection method can produce effects with the muzzle device at the same time, and it has little effect on the velocity of the projectile muzzle velocity, and it provides a new way of thinking for the development of future artillery recoil reduction technology.

2.
Arch Virol ; 169(2): 39, 2024 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-38300368

RESUMO

The complete genome sequence of a putative novel potyvirus, tentatively named "polygonatum kingianum mottle virus" (PKgMV; GenBank accession no. ON428226), infecting Polygonatum kingianum in China, was obtained by next-generation sequencing (NGS), reverse transcription polymerase chain reaction (RT-PCR), and rapid amplification of cDNA ends (RACE). PKgMV exhibits the typical genome organization and characteristics of members of the genus Potyvirus, with a length of 10,002 nucleotides (nt) and a large open reading frame (nt 108 to 9,746) encoding a polyprotein of 3,212 amino acids (aa) (363.68 kDa). Pairwise comparisons revealed that the PKgMV polyprotein shares 50.5-68.6% nt and 43.1-72.2% aa sequence identity with reported members of the genus Potyvirus. Moreover, phylogenetic analysis indicated that PKgMV is closely related to polygonatum kingianum virus 1 (PKgV1; accession no. MK427056). These results suggest that the PKgMV is a novel member of the genus Potyvirus of the family Potyviridae.


Assuntos
Polygonatum , Potyvirus , China , Filogenia , Aminoácidos , Nucleotídeos , Poliproteínas , Potyvirus/genética
3.
Sensors (Basel) ; 24(3)2024 Jan 25.
Artigo em Inglês | MEDLINE | ID: mdl-38339495

RESUMO

Photoelectric smoke detectors are the most cost-effective devices for very early warning fire alarms. However, due to the different light intensity response values of different kinds of fire smoke and interference from interferential aerosols, they have a high false-alarm rate, which limits their popularity in Chinese homes. To address these issues, an embedded spatial-temporal convolutional neural network (EST-CNN) model is proposed for real fire smoke identification and aerosol (fire smoke and interferential aerosols) classification. The EST-CNN consists of three modules, including information fusion, scattering feature extraction, and aerosol classification. Moreover, a two-dimensional spatial-temporal scattering (2D-TS) matrix is designed to fuse the scattered light intensities in different channels and adjacent time slices, which is the output of the information fusion module and the input for the scattering feature extraction module. The EST-CNN is trained and tested with experimental data measured on an established fire test platform using the developed dual-wavelength dual-angle photoelectric smoke detector. The optimal network parameters were selected through extensive experiments, resulting in an average classification accuracy of 98.96% for different aerosols, with only 67 kB network parameters. The experimental results demonstrate the feasibility of installing the designed EST-CNN model directly in existing commercial photoelectric smoke detectors to realize aerosol classification.

4.
Sci Rep ; 14(1): 586, 2024 01 05.
Artigo em Inglês | MEDLINE | ID: mdl-38182631

RESUMO

Mammalian pregnancy requires gradual yet extreme remodeling of the reproductive organs to support the growth of the embryos and their birth. After delivery, the reproductive organs return to their non-pregnant state. As pregnancy has traditionally been understudied, there are many unknowns pertaining to the mechanisms behind this remarkable remodeling and repair process which, when not successful, can lead to pregnancy-related complications such as maternal trauma, pre-term birth, and pelvic floor disorders. This study presents the first longitudinal imaging data that focuses on revealing anatomical alterations of the vagina, cervix, and uterine horns during pregnancy and postpartum using the mouse model. By utilizing advanced magnetic resonance imaging (MRI) technology, T1-weighted and T2-weighted images of the reproductive organs of three mice in their in vivo environment were collected at five time points: non-pregnant, mid-pregnant (gestation day: 9-10), late pregnant (gestation day: 16-17), postpartum (24-72 h after delivery) and three weeks postpartum. Measurements of the vagina, cervix, and uterine horns were taken by analyzing MRI segmentations of these organs. The cross-sectional diameter, length, and volume of the vagina increased in late pregnancy and then returned to non-pregnant values three weeks after delivery. The cross-sectional diameter of the cervix decreased at mid-pregnancy before increasing in late pregnancy. The volume of the cervix peaked at late pregnancy before shortening by 24-72 h postpartum. As expected, the uterus increased in cross-sectional diameter, length, and volume during pregnancy. The uterine horns decreased in size postpartum, ultimately returning to their average non-pregnant size three weeks postpartum. The newly developed methods for acquiring longitudinal in vivo MRI scans of the murine reproductive system can be extended to future studies that evaluate functional and morphological alterations of this system due to pathologies, interventions, and treatments.


Assuntos
Imageamento por Ressonância Magnética , Útero , Feminino , Humanos , Gravidez , Animais , Camundongos , Útero/diagnóstico por imagem , Projetos de Pesquisa , Vagina/diagnóstico por imagem , Período Pós-Parto , Mamíferos
5.
Adv Mater ; 36(6): e2307726, 2024 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-37775103

RESUMO

Diseases in pregnancy endanger millions of fetuses worldwide every year. The onset of these diseases can be early warned by the dynamic abnormalities of biochemicals in amniotic fluid, thus requiring real-time monitoring. However, when continuously penetrated by detection devices, the amnion is prone to loss of robustness and rupture, which is difficult to regenerate. Here, an interface-stabilized fiber sensor is presented for real-time monitoring of biochemical dynamics in amniotic fluid during pregnancy. The sensor is seamlessly integrated into the amnion through tissue adhesion, amniotic regeneration, and uniform stress distribution, posing no risk to the amniotic fluid environment. The sensor demonstrates a response performance of less than 0.3% fluctuation under complex dynamic conditions and an accuracy of more than 98% from the second to the third trimester. By applying it to early warning of diseases such as intrauterine hypoxia, intrauterine infection, and fetal growth restriction, fetal survival increases to 95% with timely intervention.


Assuntos
Âmnio , Líquido Amniótico , Gravidez , Feminino , Humanos
6.
Sci Rep ; 13(1): 20969, 2023 11 28.
Artigo em Inglês | MEDLINE | ID: mdl-38017264

RESUMO

Hepatocellular carcinoma (HCC) is a lethal malignancy worldwide with an increasing number of new cases each year. Apolipoprotein (APOL) isoforms have been explored for their associations with HCC.The GSE14520 cohort was used for training data; The Cancer Genome Atlas (TCGA) database was used for validated data. Diagnostic, prognostic significance and mechanisms were explored using these cohorts. Risk score models and nomograms were constructed using prognosis-related isoforms and clinical factors for survival prediction. Oncomine and HCCDB databases were further used for validation of diagnostic, prognostic significance. APOL1, 3, and 6 were differentially expressed in two cohorts (all P ≤ 0.05). APOL1 and APOL6 had diagnostic capacity whereas APOL3 and APOL6 had prognostic capacity in two cohorts (areas under curves [AUCs] > 0.7, P ≤ 0.05). Mechanism studies demonstrated that APOL3 and APOL6 might be involved in humoral chemokine signaling pathways (all P ≤ 0.05). Risk score models and nomograms were constructed and validated for survival prediction of HCC. Moreover, diagnostic values of APOL1 and weak APOL6 were validated in Oncomine database (AUC > 0.700, 0.694); prognostic values of APOL3 and APOL6 were validated in HCCDB database (all P < 0.05). Differentially expressed APOL1 and APOL6 might be diagnostic biomarkers; APOL3 and APOL6 might be prognostic biomarkers of RFS and OS for HCC via chemokine signaling pathways.


Assuntos
Carcinoma Hepatocelular , Neoplasias Hepáticas , Humanos , Carcinoma Hepatocelular/diagnóstico , Carcinoma Hepatocelular/genética , Apolipoproteína L1/genética , Neoplasias Hepáticas/diagnóstico , Neoplasias Hepáticas/genética , Isoformas de Proteínas , Biomarcadores , Quimiocinas , Prognóstico
7.
Front Immunol ; 14: 1269451, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-37868994

RESUMO

Regulation of cell mortality for disease treatment has been the focus of research. Ferroptosis is an iron-dependent regulated cell death whose mechanism has been extensively studied since its discovery. A large number of studies have shown that regulation of ferroptosis brings new strategies for the treatment of various benign and malignant diseases. Iron excess and lipid peroxidation are its primary metabolic features. Therefore, genes involved in iron metabolism and lipid metabolism can regulate iron overload and lipid peroxidation through direct or indirect pathways, thereby regulating ferroptosis. In addition, glutathione (GSH) is the body's primary non-enzymatic antioxidants and plays a pivotal role in the struggle against lipid peroxidation. GSH functions as an auxiliary substance for glutathione peroxidase 4 (GPX4) to convert toxic lipid peroxides to their corresponding alcohols. Here, we reviewed the researches on the mechanism of ferroptosis in recent years, and comprehensively analyzed the mechanism and regulatory process of ferroptosis from iron metabolism and lipid metabolism, and then described in detail the metabolism of GPX4 and the main non-enzymatic antioxidant GSH in vivo.


Assuntos
Ferroptose , Sobrecarga de Ferro , Humanos , Fosfolipídeo Hidroperóxido Glutationa Peroxidase/metabolismo , Ferro/metabolismo , Glutationa Peroxidase/metabolismo , Peroxidação de Lipídeos/fisiologia , Antioxidantes/metabolismo , Glutationa/metabolismo
8.
Sci Rep ; 13(1): 16859, 2023 10 06.
Artigo em Inglês | MEDLINE | ID: mdl-37803063

RESUMO

Worldwide, cancer is a huge burden, and each year sees an increase in its incidence. RAB (Ras-related in brain) 13 is crucial for a number of tumor types. But more research on RAB13's tumor-related mechanism is still required. This study's goal was to investigate RAB13's function in human pan-cancer, and we have also preliminarily explored the relevant mechanisms. To investigate the differential expression, survival prognosis, immunological checkpoints, and pathological stage of RAB13 in human pan-cancer, respectively, databases of TIMER2.0, GEPIA 2, and UALCAN were employed. CBioPortal database was used to analyze the mutation level, meanwhile, PPI network was constructed based on STRING website. The putative functions of RAB13 in immunological infiltration were investigated using single sample gene set enrichment analysis (ssGSEA). The mechanism of RAB13 in hepatocellular cancer was also briefly investigated by us using gene set enrichment analysis (GSEA). RAB13 was differentially expressed in a number of different cancers, including liver hepatocellular carcinoma (LIHC), stomach adenocarcinoma (STAD), etc. Additionally, RAB13 overexpression in LGG and LIHC is associated with a worse prognosis, including overall survival (OS) and disease-free survival (DFS). Then, we observed that early in BLCA, BRAC, CHOL, ESCA, HNSC, KICH, KIRC, LIHC, LUAD, LUSC, and STAD, the level of RAB13 expression was raised. Next, we found that "amplification" was the most common mutation in RAB13. The expression of SLC39A1, JTB, SSR2, SNAPIN, and RHOC was strongly positively linked with RAB13, according to a correlation study. RAB13 favorably regulated B cell, CD8 + T cell, CD4 + T cell, macrophage, neutrophil, and dendritic cell in LIHC, according to immune infiltration analysis. Immune checkpoint study revealed a positive correlation between RAB13 expression and PD1, PDL1, and CTLA4 in LIHC. According to GSEA, RAB13 is involved in a number of processes in LIHC, including MTORC1 signaling, MYC targets v1, G2M checkpoint, MITOTIC spindle, DNA repair, P53 pathway, glycolysis, PI3K-AKT-MTOR signaling, etc. RAB13 is a possible therapeutic target in LIHC and can be used as a prognostic marker.


Assuntos
Adenocarcinoma , Carcinoma Hepatocelular , Neoplasias Hepáticas , Neoplasias Gástricas , Humanos , Fosfatidilinositol 3-Quinases , Carcinoma Hepatocelular/genética , Proteínas rab de Ligação ao GTP/genética
9.
bioRxiv ; 2023 Oct 16.
Artigo em Inglês | MEDLINE | ID: mdl-37693372

RESUMO

Dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) is a routine method to non-invasively quantify perfusion dynamics in tissues. The standard practice for analyzing DCE-MRI data is to fit an ordinary differential equation to each voxel. Recent advances in data science provide an opportunity to move beyond existing methods to obtain more accurate measurements of fluid properties. Here, we developed a localized convolutional function regression that enables simultaneous measurement of interstitial fluid velocity, diffusion, and perfusion in 3D. We validated the method computationally and experimentally, demonstrating accurate measurement of fluid dynamics in situ and in vivo. Applying the method to human MRIs, we observed tissue-specific differences in fluid dynamics, with an increased fluid velocity in breast cancer as compared to brain cancer. Overall, our method represents an improved strategy for studying interstitial flows and interstitial transport in tumors and patients. We expect that it will contribute to the better understanding of cancer progression and therapeutic response.

10.
Heliyon ; 9(6): e16719, 2023 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-37303532

RESUMO

This study identified a novel virus in the family Partitiviridae infecting Polygonatum kingianum Coll. et Hemsl, which is tentatively named polygonatum kingianum cryptic virus 1 (PKCV1). PKCV1 genome has two RNA segments: dsRNA1 (1926 bp) has an open reading frame (ORF) encoding an RNA-dependent RNA polymerase (RdRp) of 581 amino acids (aa), and dsRNA2 (1721 bp) has an ORF encoding a capsid protein (CP) of 495 aa. The RdRp of PKCV1 shares 20.70-82.50% aa identity with known partitiviruses, and the CP of PKCV1 shares 10.70-70.80% aa identity with known partitiviruses. Moreover, PKCV1 phylogenetically clustered with unclassified members of the Partitiviridae family. Additionally, PKCV1 is common in P. kingianum planting regions and has a high infection rate in P. kingianum seeds.

11.
Carbohydr Polym ; 317: 121086, 2023 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-37364956

RESUMO

Demyelination is a serious complication of neurological disorders, which can be reversed by oligodendrocyte precursor cell (OPC) as the available source of myelination. Chondroitin sulfate (CS) plays key roles in neurological disorders, which still attracted less attention on how CS modulates the fate of OPCs. Nanoparticle coupled with glycoprobe is a potential strategy for investigating the carbohydrate-protein interaction. However, there is lack of CS-based glycoprobe with enough chain length that interact with protein effectively. Herein, we designed a responsive delivery system, in which CS was the target molecule, and cellulose nanocrystal (CNC) was the penetrative nanocarrier. A coumarin derivative (B) was conjugated at the reducing end of an unanimal-sourced chondroitin tetrasaccharide (4mer). This glycoprobe (4B) was grafted to the surface of a rod-like nanocarrier, which had a crystalline core and a poly(ethylene glycol) shell. This glycosylated nanoparticle (N4B-P) displayed a uniform size, improved water-solubility, and responsive release of glycoprobe. N4B-P displayed strong green fluorescence and good cell-compatibility, which imaged well the neural cells including astrocytes and OPCs. Interestingly, both of glycoprobe and N4B-P were internalized selectively by OPCs when they were incubated in astrocytes/OPCs mixtures. This rod-like nanoparticle would be a potential probe for studying carbohydrate-protein interaction in OPCs.


Assuntos
Nanopartículas , Células Precursoras de Oligodendrócitos , Oligodendroglia/metabolismo , Diferenciação Celular , Celulose/farmacologia , Celulose/metabolismo , Sulfatos de Condroitina/metabolismo
12.
Biomimetics (Basel) ; 8(1)2023 Mar 09.
Artigo em Inglês | MEDLINE | ID: mdl-36975342

RESUMO

The quadruped robot has a strong motion performance and broad application prospects in practical applications. However, during the movement of the quadruped robot, it is easy to be affected by external disturbance and environmental changes, which makes it unable to achieve the ideal effect movement. Therefore, it is very important for the quadruped robot to adjust actively according to its own state detection. This paper proposes an active state adjustment control method based on its own state, which can realize disturbance recovery and active environment adaptation. Firstly, the controller is designed according to the physical model of the quadruped robot, and the foot forces are optimized using the quadratic program (QP) method. Then, the disturbance compensation method based on dynamic analysis is studied and combined with the controller itself. At the same time, according to the law of biological movement, the movement process of the quadruped robot is actively adjusted according to the different movement environment, so that it can adapt to various complex environments. Finally, it is verified in a simulation environment and quadruped robot prototype. The results show that the quadruped robot has a strong active disturbance recovery ability and active environment adaptability.

13.
RSC Adv ; 12(46): 29852-29864, 2022 Oct 17.
Artigo em Inglês | MEDLINE | ID: mdl-36321088

RESUMO

The rheological and filtration performance of drilling fluids greatly depends on the additives used. To address the negative impact on the drilling fluid performance stemming from electrolyte contamination, a sustainable sodium alginate (SA) biopolymer was employed as an additive in water-based drilling fluids to overcome the performance deterioration caused by the polyelectrolyte effect under salt contamination. The results demonstrated that SA performs better than sodium carboxymethyl cellulose (Na-CMC) and polyanionic cellulose (PAC-LV), the widely used drilling fluid additives. Although exposed to highly concentrated salt contamination, the addition of SA can mitigate viscosity variation and maintain a lower filtration volume of a base fluid (BF), whereas an advanced variation in CMC/BF and PAC/BF was observed. The possible rheology and filtration mechanism of SA under highly concentrated salt contamination were investigated through zeta potential, particle size distribution, and scanning electron microscopy (SEM). The results revealed that the anchoring groups on the SA molecular chain enable them to strongly adsorb on the negatively charged bentonite surface via hydrogen and ionic bond interactions, leading to a significant improvement in both rheological and filtration performance. Therefore, SA with excellent salt tolerance and sustainability confers practical applicability that could extend to the preparation of saltwater-based and other inhibitive drilling fluids.

14.
Langmuir ; 38(41): 12539-12550, 2022 Oct 18.
Artigo em Inglês | MEDLINE | ID: mdl-36213955

RESUMO

Wettability alteration of the shale surface is a potential strategy to address wellbore instability issues arising from shale hydration. In this study, we have explored an oil-in-water (o/w) nanoemulsion, in which soluble silicate (lithium silicate and potassium methyl silicate) as the aqueous phase and organosilanes (3-methacryloxypropyltrimethoxysilane (KH570) and n-octyltriethoxysilane (n-OTES)) as the oil phase, as a shale inhibitor via forming a hydrophobic "artificial borehole shield" in situ on shale surfaces to maintain wellbore stability in high-temperature drilling operations. The shale dispersion test showed the highest shale recovery of nanoemulsion was up to 106.4% compared to that of water (20%), and recovered shale cuttings remained at the original integrity after hot rolling at 180 °C, indicating superior inhibition performance and resistance to elevated temperatures. Moreover, recovered shale cuttings manifested water repellency upon reimmersion in water, ascribed to the hydrophobic film, preventing water from permeating into the shale. The results of the contact angle measurement elucidated that the film wettability, from hydrophilic to superhydrophobic (ranging from 9.6-154°), can be achieved by altering the n-OTES-to-KH570 weight ratio from 0.2 to 2.25, and the film with the highest hydrophobicity (154°) and the lowest surface energy (3.17 mJ·m-2) can be obtained at a ratio of 1.3. Scanning electron microscopy images demonstrated that the superhydrophobic film was composed of tightly stacked reticulate nanofilaments with a diameter of 7-17 nm and several micrometers in length and overlapped well-distributed nanospheres with a diameter of 30 nm. X-ray diffraction and Fourier transform infrared spectroscopy confirmed the film was crystalline silica grafted with long-chain alkylsiloxane. It is assumed that the unique micronanostructure combined with the siloxane modification contributed to the hydrophobicity. Consequently, this study provides a potential alternative solution for wellbore stabilization in deep well drilling engineering by employing nanoemulsion as a shale hydration inhibitor via forming a protective film with controllable wettability. Furthermore, it can be conferred a practical application due to easily available, less hazardous, and cost-effective materials.

15.
ACS Omega ; 6(31): 20577-20589, 2021 Aug 10.
Artigo em Inglês | MEDLINE | ID: mdl-34396003

RESUMO

Effective control of shale swelling and lost circulation using drilling fluid is considered the dominant strategy for maintaining borehole stability, especially drilling operations in deep oil and gas wells. In this work, a hybrid silicate that contains lithium silicate and potassium methyl silicate (PMS) was employed as a film-forming additive to reduce shale hydration and filtration loss in the high-temperature drilling fluid. Scanning electron microscopy (SEM) results revealed that a dense quartz crystal film coating on the shale can be formed in a hybrid silicate solution when the temperature exceeds 150 °C. The in situ-formed film on the shale surface with a thickness of 60-130 µm was composed of fibrous crystalline silica. Furthermore, the aqueous hybrid silicate exhibited enhanced hydration inhibition ability by preventing water invasion of the formation. Aqueous hybrid silicate with a concentration of 0.5-3 wt % lithium silicate and 0.1-0.2 mol/L PMS was first chosen to obtain the optimum concentration according to the hydration inhibition ability and film formation characteristics. The hybrid silicate was added into a drilling fluid formulation applicable in high-pressure and high-temperature conditions, and the rheological characteristics and filtration properties were investigated. The results confirmed that drilling fluids with the addition of hybrid silicate can mitigate variation of viscosity and yield point before and after aging at 180 and 220 °C. Besides, the filtration behavior was also improved by adding hybrid silicate into the drilling fluid. A lower filtration loss was observed at the concentration of 1.0 wt % lithium silicate and 0.2 mol/L potassium methyl silicate, which showed 63 and 50% HPHT fluid loss reduction for unweighted and weighted formulations at 205 °C and 3.5 MPa, respectively. In addition, the drilling fluid featured stable rheological and filtration properties and excellent shale hydration inhibition characteristics when exposed to high temperatures, making it a promising candidate for drilling in deep oil and gas wells.

16.
ACS Appl Mater Interfaces ; 13(17): 20024-20033, 2021 May 05.
Artigo em Inglês | MEDLINE | ID: mdl-33900745

RESUMO

Exploring electrocatalysts with satisfactory activity and durability has remained a long-lasting target for electrolyzing water, which is particularly significant for sustainable hydrogen fuel production. Here, we report a quaternary B/P-codoped transition metal Co-Mo hybrid as an efficient alternative catalyst for overall water splitting. The Co-Mo-B-P/CF dual nanowafers were deposited on a copper foam by double-pulse electrodeposition, which is favorable for achieving a nanocrystalline structure. The Co-Mo-B-P/CF catalyst shows a high catalytic activity along with good long-term stability in 1.0 M KOH solutions for both the hydrogen and oxygen evolution reactions, requiring 48 and 275 mV to reach 10 mA cm-2, respectively. The synergetic effect between Co-Mo and doped B and P elements is mainly attributed to the excellent bifunctional catalysis performance, while the dual-nanowafer structure endows Co-Mo-B-P with numerous catalytical active sites enhancing the utilization efficiency of atoms. Moreover, the catalytic capability of Co-Mo-B-P/CF as a bifunctional electrocatalyst for the overall water splitting is proved, with the current density of 10 mA cm-2 accomplished at 1.59 V. After the stability test for overall water splitting at 1.59 V for 24 h, the activity almost remains unchanged. The features of excellent electrocatalytic activity, simple preparation, and inexpensive raw materials for Co-Mo-B-P/CF as a bifunctional catalyst hold great potentials for overall water splitting.

17.
Front Cell Neurosci ; 15: 807170, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-35027884

RESUMO

Abnormalities in the prefrontal cortex (PFC), as well as the underlying white matter (WM) tracts, lie at the intersection of many neurodevelopmental disorders. The influence of microorganisms on brain development has recently been brought into the clinical and research spotlight as alterations in commensal microbiota are implicated in such disorders, including autism spectrum disorders, schizophrenia, depression, and anxiety via the gut-brain axis. In addition, gut dysbiosis is common in preterm birth patients who often display diffuse WM injury and delayed WM maturation in critical tracts including those within the PFC and corpus callosum. Microbial colonization of the gut aligns with ongoing postnatal processes of oligodendrogenesis and the peak of brain myelination in humans; however, the influence of microbiota on gyral WM development remains elusive. Here, we develop and validate a neonatal germ-free swine model to address these issues, as piglets share key similarities in WM volume, developmental trajectories, and distribution to humans. We find significant region-specific reductions, and sexually dimorphic trends, in WM volume, oligodendrogenesis, and mature oligodendrocyte numbers in germ-free piglets during a key postnatal epoch of myelination. Our findings indicate that microbiota plays a critical role in promoting WM development during early life when the brain is vulnerable to environmental insults that can result in an array of disabilities manifesting later in life.

18.
J Cereb Blood Flow Metab ; 40(10): 2098-2114, 2020 10.
Artigo em Inglês | MEDLINE | ID: mdl-31696765

RESUMO

High-resolution fMRI enables noninvasive mapping of the hemodynamic responses from individual penetrating vessels in animal brains. Here, a 2D multi-echo single-vessel fMRI (MESV-fMRI) method has been developed to map the fMRI signal from arterioles and venules with a 100 ms sampling rate at multiple echo times (TE, 3-30 ms) and short acquisition windows (<1 ms). The T2*-weighted signal shows the increased extravascular effect on venule voxels as a function of TE. In contrast, the arteriole voxels show an increased fMRI signal with earlier onset than venules voxels at the short TE (3 ms) with increased blood inflow and volume effects. MESV-fMRI enables vessel-specific T2* mapping and presents T2*-based fMRI time courses with higher contrast-to-noise ratios (CNRs) than the T2*-weighted fMRI signal at a given TE. The vessel-specific T2* mapping also allows semi-quantitative estimation of the oxygen saturation levels (Y) and their changes (ΔY) at a given blood volume fraction upon neuronal activation. The MESV-fMRI method enables vessel-specific T2* measurements with high spatiotemporal resolution for better modeling of the fMRI signal based on the hemodynamic parameters.


Assuntos
Mapeamento Encefálico/métodos , Circulação Cerebrovascular/fisiologia , Imageamento por Ressonância Magnética/métodos , Algoritmos , Animais , Arteríolas/diagnóstico por imagem , Volume Sanguíneo , Imagem Ecoplanar , Processamento de Imagem Assistida por Computador , Masculino , Consumo de Oxigênio , Ratos , Ratos Sprague-Dawley , Vênulas/diagnóstico por imagem
19.
ACS Appl Mater Interfaces ; 11(33): 29708-29717, 2019 Aug 21.
Artigo em Inglês | MEDLINE | ID: mdl-31347824

RESUMO

In order to reduce the charge-transfer resistance, ohmic resistance, and ionic and electronic resistances arising from the polymer binder, designing and constructing self-standing and binder-free porous electrodes are very significant for energy storage and conversion devices. Herein, self-standing and binder-free porous N-Co carbon nanofiber (N-Co/CNF) cathodes are prepared for zinc-air batteries (ZABs) by an in situ electrospinning/plasma-etching method. The morphology and activity of the prepared electrodes are investigated by several characterization techniques. The prepared specimens exhibit a multilayered CNF structure, and a new CoN compound is produced after plasma-etching treatment. The N-Co/CNF-300-10 cathode demonstrates excellent electrocatalytic performance toward oxygen reduction reaction, with an onset potential and a half-wave potential of 0.995 and 0.853 V (vs reversible hydrogen electrode), respectively, which is comparable to that of 20% Pt/C. The N-Co/CNF-300-10 cathode acting as a self-standing electrode for ZABs exhibits a maximum discharge power density as high as 229 mW cm-2 and a specific capacity of 659.6 mA h gZn-1, which are much higher than those of the commercial catalysts, benefiting from the self-standing porous structure, N-doping, and more defects and active sites induced by plasma-etching. It provides an effective way to construct a self-standing porous electrode with controllable compositions for rechargeable metal-air batteries.

20.
Materials (Basel) ; 12(10)2019 May 16.
Artigo em Inglês | MEDLINE | ID: mdl-31100797

RESUMO

SiCp/6061Al composites have been developed and widely applied in many fields, such as automobile, aerospace, shipping, and so on. Considering heat treatment, service environment, and strength of composites, this paper comprehensively studies the mechanical properties of SiCp/6061Al composites with a large range of SiC mass fractions under T6 treatments and different tensile temperatures. SiCp/6061Al composites were successfully prepared by hot press sintering at various SiC mass fractions (0-30%), and the influences of SiC concentration and T6 treatment on the mechanical properties of composites were characterized via tensile tests at room temperature, 100, and 200 °C. Microstructure and fracture surfaces of composites with various SiC concentrations were further analyzed by optical microscope and SEM. The formula for the biggest critical reinforcement concentration for the saturated distribution of SiC is proposed to reveal the strengthening rule of different SiC concentrations. Results show that the effect of T6 treatment on the mechanical properties of composites is a marked increase in tensile strength and an obvious decrease in elongation. The increase in the SiC mass fraction, except at 30%, is able to bring an increase in tensile strength and a decrease in elongation, and the change of the elongation is insignification in T6-treated specimens. The tensile strength of T6-treated specimens decreases as temperature increases, and the composite has a maximum elongation at 100 °C.

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